Journal: Bioactive Materials

Article Title: Mesenchymal stromal cells-loaded 3D radially aligned composite scaffold with potentiated paracrine signaling for sequential bone regeneration

doi: 10.1016/j.bioactmat.2026.02.059

Figure Lengend Snippet: Temporal analysis of the BMSC paracrine profile on different scaffolds. (A) Confocal microscopy images from Live/Dead fluorescence staining of BMSCs encapsulated within the PCL/HAp-GelMA/BMSCs scaffold after 1, 3, 5, and 14 d of 3D culture (live cells, green; dead cells, red). (B) The concentrations of key paracrine factors (TGF-β, PGE2, VEGF, HGF, and BMP-2) from BMSCs cultured in different scaffolds, quantified from culture supernatants at day 3 and day 7. (C) Corresponding relative mRNA expression levels of TGFB1, PTGS2, VEGFA, HGF, and BMP-2 in BMSCs at day 3 and day 7, as determined by qPCR analysis. Data are presented as mean ± SD (n = 3) *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001; ns: not significant.

Article Snippet: ELISA kits for PGE2 (Cat. No. E-EL-0034), TGF-β (Cat. No. E-EL-0162), VEGF (Cat. No. E-EL-R2603), and HGF (Cat. No. E-EL-R0496) were purchased from Elabscience (Wuhan, China).

Techniques: Confocal Microscopy, Fluorescence, Staining, Cell Culture, Expressing

Journal: Neural Regeneration Research

Article Title: Small extracellular vesicles derived from hair follicle neural crest stem cells enhance perineurial cell proliferation and migration via the TGF-β/SMAD/HAS2 pathway

doi: 10.4103/NRR.NRR-D-25-00127

Figure Lengend Snippet: miR-21-5p activates the TGF-β/SMAD pathway by inhibiting SMAD7, resulting in upregulated HAS2 and increased PC proliferation and migration. (A, B) The volcano plot (A) and heatmap (B) illustrated the differential gene expression in regenerated tissue between the phosphate-buffered saline (PBS) and small extracellular vesicles derived from hair follicle neural crest stem cells (hfNCSC-sEVs) groups on day 7 post-operation ( n = 3 per group). (C) The chord diagram of the Gene Ontology enrichment analysis and (D) bubble chart of the Kyoto Encyclopedia of Genes and Genomes enrichment analysis for differential genes highlighted the enriched functions and associated pathways. (E) The pie chart displayed the top 10 most abundant microRNAs (miRNAs) in hfNCSC-sEVs. (F) Schematic representation of the predicted binding sites of miR-21-5p on its target gene Smad7 , as identified by TargetScan. (G) Quantitative reverse transcription-polymerase chain reaction results indicated the relative expression levels of miR-21-5p in PCs from the PBS and hfNCSC-sEVs groups on day 3 of in vitro culture ( n = 3 per group). (H) The dual-luciferase reporter assay demonstrated the relative luciferase activity following the co-transfection of reporter constructs from the Control, wild-type (WT) Smad7 3′ untranslated region (UTR), and mutant Smad7 3′ UTR groups with either miR-21-5p overexpression plasmid or negative control vector ( n = 3 per group). (I, J) Western blot (I) and statistical analysis (J) revealed the relative protein expression levels of SMAD7 and HAS2, as well as the phosphorylation levels of SMAD2/3 in PCs from the –/–, TGF-β/–, and TGF-β/miR-21-5p groups on day 5 of in vitro culture (normalized to β-actin, n = 3 per group). Data are expressed as the mean ± SEM. ** P < 0.01, *** P < 0.001 (Student’s t -test for G; one-way analysis of variance and Tukey’s multiple comparison test for J, H). The data were from at least three separate and independent studies. GO: Gene Ontology; HAS2: hyaluronan synthase 2; hfNCSCs: hair follicle neural crest stem cells; KEGG: Kyoto Encyclopedia of Genes and Genomes; MUT: mutant; NC: negative control; ns: not significant; PCs: perineurial cells; sEVs: small extracellular vesicles; SMAD: mothers against decapentaplegic homolog; TGF-β: transforming growth factor-beta; WT: wild type.

Article Snippet: For cell treatment, transforming growth factor (TGF)-β (Cat# HY- P70648 , MedChemExpress, Monmouth Junction, NJ, USA) was administered at a concentration of 5 ng/mL. miR-21-5p mimics or si- Has2 , provided by Sangon Biotech Co., Ltd., were transfected using Lipofectamine RNAiMAX reagent (Cat# L13778150, Invitrogen) according to the instructions.

Techniques: Migration, Gene Expression, Saline, Derivative Assay, Binding Assay, Reverse Transcription, Polymerase Chain Reaction, Expressing, In Vitro, Luciferase, Reporter Assay, Activity Assay, Cotransfection, Construct, Control, Mutagenesis, Over Expression, Plasmid Preparation, Negative Control, Western Blot, Phospho-proteomics, Comparison

Journal: Molecular and Clinical Oncology

Article Title: Long non-coding RNAs affect the metastasis of hepatocellular carcinoma cells by regulating the epithelial-to-mesenchymal transition

doi: 10.3892/mco.2026.2940

Figure Lengend Snippet: Validation of the epithelial-to-mesenchymal transition model. (A) Micrographs of Huh7 cells treated with or without 10 ng/ml TGF-β for 4 days. (B) Relative mRNA levels of E-cadherin, N-cadherin, Snail and Slug in Huh7 cells treated with or without 10 ng/ml TGF-β for 4 days. (C) In vitro migration of Huh7 cells treated with or without 10 ng/ml TGF-β for 4 days. * P<0.05 vs. the control group. TGF-β, transforming growth factor-β.

Article Snippet: To induce the in vitro EMT cell model, Huh7 cells were cultured at 37 ̊C in DMEM with 2.5% FBS and 10 ng/ml TGF-β (cat. no. HY-P7118; MedChemExpress) for 4 days.

Techniques: Biomarker Discovery, In Vitro, Migration, Control

Journal: Molecular and Clinical Oncology

Article Title: Long non-coding RNAs affect the metastasis of hepatocellular carcinoma cells by regulating the epithelial-to-mesenchymal transition

doi: 10.3892/mco.2026.2940

Figure Lengend Snippet: DE lncRNAs and mRNAs in TGF-β treated Huh7 cells. (A) Volcano plot demonstrating DE lncRNAs and mRNAs. Red points represent upregulated RNAs, blue points represent downregulated RNAs and black points represent RNAs with no significant differences. (B) Hierarchical clustering analysis based on the significantly DE lncRNAs and mRNAs. Red indicates high relative expression levels, blue indicates low relative expression levels and white indicates no change in the gene expression levels. The color brightness indicates the extent of the upregulation or downregulation of the gene expression. TGF-β, transforming growth factor-β; DE, differentially expressed; lncRNA, long non-coding RNA; FC, fold-change; FDR, false-discovery rate.

Article Snippet: To induce the in vitro EMT cell model, Huh7 cells were cultured at 37 ̊C in DMEM with 2.5% FBS and 10 ng/ml TGF-β (cat. no. HY-P7118; MedChemExpress) for 4 days.

Techniques: Expressing, Gene Expression

Journal: Molecular and Clinical Oncology

Article Title: Long non-coding RNAs affect the metastasis of hepatocellular carcinoma cells by regulating the epithelial-to-mesenchymal transition

doi: 10.3892/mco.2026.2940

Figure Lengend Snippet: GO and KEGG enrichment analysis. GO term enrichment categories, including (A) molecular function, (B) cellular component and (C) biological process. (D) KEGG pathway enrichment analysis. GO, Gene Ontology; KEGG, Kyoto Encyclopedia of Genes and Genomes; ECM, extracellular matrix; TGF-β, transforming growth factor-β; PPAR, peroxisome proliferator-activated receptor; AGE-RAGE, advanced glycation end-products-receptor for advanced glycation end-products.

Article Snippet: To induce the in vitro EMT cell model, Huh7 cells were cultured at 37 ̊C in DMEM with 2.5% FBS and 10 ng/ml TGF-β (cat. no. HY-P7118; MedChemExpress) for 4 days.

Techniques:

Journal: Molecular and Clinical Oncology

Article Title: Long non-coding RNAs affect the metastasis of hepatocellular carcinoma cells by regulating the epithelial-to-mesenchymal transition

doi: 10.3892/mco.2026.2940

Figure Lengend Snippet: Validation of the sequencing data using reverse transcription-quantitative PCR. (A) Relative mRNA levels of COL1A1, BMP6, TUBA1A, ATP2B2 and F2 in Huh7 cells treated with or without 10 ng/ml TGF-β for 4 days. (B) Relative lncRNA levels of NNMT-205, CASC15-204, UBASH3B-202, CAPN2-206, CAV2-214 and ZSWIM8-210 in Huh7 cells treated with or without 10 ng/ml TGF-β for 4 days. * P<0.05 vs. the control group. lncRNA, long non-coding RNA; TGF-β, transforming growth factor-β.

Article Snippet: To induce the in vitro EMT cell model, Huh7 cells were cultured at 37 ̊C in DMEM with 2.5% FBS and 10 ng/ml TGF-β (cat. no. HY-P7118; MedChemExpress) for 4 days.

Techniques: Biomarker Discovery, Sequencing, Reverse Transcription, Real-time Polymerase Chain Reaction, Control